Entomology Research Today is a free monthly online journal that collates and summarizes the latest research about Entomology, including details on insects, parasites, diseases.

A comparative analysis of serpin genes in the silkworm genome.

Zou Z, Picheng Z, Weng H, Mita K, Jiang H

Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK 74078, USA.

Serine protease inhibitors (serpins) are a superfamily of proteins, most of which control protease-mediated processes by inhibiting their cognate enzymes. Sequencing of the silkworm genome provides an opportunity to investigate serpin structure, function, and evolution at the genome level. There are thirty-four serpin genes in Bombyx mori. Six are highly similar to their Manduca sexta orthologs that regulate innate immunity. Three alternative exons in serpin1 gene and four in serpin28 encode a variable region including the reactive site loop. Splicing of serpin2 pre-mRNA yields variations in serpin2A, 2A' and 2B. Sequence similarity and intron positions reveal the evolutionary pathway of seven serpin genes in group C. RT-PCR indicates an increase in the mRNA levels of serpin1, 3, 5, 6, 9, 12, 13, 25, 27, 32 and 34 in fat body and hemocytes of larvae injected with bacteria. These results suggest that the silkworm serpins play regulatory roles in defense responses.

Published 16 March 2009 in Genomics, 93(4): 367-75.
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